GSM1811775: NT H3K9me3 ChIP-Seq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9me3
Cell type
Cell type Class
Embryo
Cell type
2-cell stage
NA
NA
Attributes by original data submitter
Sample
source_name
NT H3K9me3 ChIP-seq
strain background
B6D2F1 (C57BL/6 X DBA/2)
development stage
2-cell
genotype/variation
wild type
tissue
nuclear transfer embryo
chip antibody
H3K9me3
chip antibody vendor
active motif
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
500-1000 cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to illumina TruSeq DNA LT Sample Prep Kit( FC-121-2001, FC-121-2002)