Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Gonad

Cell type

Cumulus Cells

MeSH Description

The granulosa cells of the cumulus oophorus which surround the OVUM in the GRAAFIAN FOLLICLE. At OVULATION they are extruded with OVUM.

Sample

source_name

CC H3K9me3 ChIP-seq

strain background

B6D2F1 (C57BL/6 X DBA/2)

development stage

6-8 weeks post natal

genotype/variation

wild type

tissue

cumulus cell

chip antibody

H3K9me3

chip antibody vendor

active motif

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

500-1000 cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to illumina TruSeq DNA LT Sample Prep Kit( FC-121-2001, FC-121-2002)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

12563373

Reads aligned (%)

61.0

Duplicates removed (%)

50.3

Number of peaks

90 (qval < 1E-05)

mm9

Number of total reads

12563373

Reads aligned (%)

60.6

Duplicates removed (%)

50.8

Number of peaks

66 (qval < 1E-05)