GSM1811341: C2 HA ChIPSeq; Drosophila melanogaster; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Adult
Cell type
Adult female
NA
NA
Attributes by original data submitter
Sample
source_name
Female whole animal_control_no_paraquat
tissue
whole animal
gender
female
age
1-3 days
genotype
k6801/k6801;gHA-KDM5
chip antibody
HA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
To identify KDM5 binding sites, ChIP-seq was carried out using HA-tagged KDM5 genomic rescue transgene flies with anti-HA (with or without paraquat treatment for 6 hours). Wildtype w1118 adults were used for KDM5 and anti-H3K4me3 ChIP-seq. Libraries were prepared using Tru-Seq adaptors and then size-selected (150 bp-350 bp) and purified by agarose gel