Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
prostate cancer cell line
cell line
LNCaP
sirna
siTRIM33 #1
treatment
EtOH for 2 h
chip antibody
AR (Santa Cruz, sc-H280)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and interested protein-DNA complexes were isolated with antibody. Library preparations were constructed using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® according to the manufacturer's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
66364829
Reads aligned (%)
88.7
Duplicates removed (%)
72.3
Number of peaks
2506 (qval < 1E-05)

hg19

Number of total reads
66364829
Reads aligned (%)
88.0
Duplicates removed (%)
74.2
Number of peaks
2423 (qval < 1E-05)

Base call quality data from DBCLS SRA