SpDamID used Dneasy extraction kit, DpnI digestion, and ligation mediated PCR to generate libraries that are in turn sonicated and ligated to bar-coded Illumina sequencing adaptors; FAIRE was performed by sonicating formaldehyde cross-linked nuclei and phenol:chloroform extraction of open chromatin compared to input DNA extracted after cross-link reversal, ChIP used myc-epitope tag to immunoprecipitate bound DNA from sonicated cross-linked DNA Illumina library construction protocol