Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Bisulfite-Seq
Antigen
Bisulfite-Seq

Cell type

Cell type Class
Lung
Cell type
Lung
MeSH Description
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.

Attributes by original data submitter

Sample

source_name
Lung
strain background
C57BL/6N
age
7 weeks
genotype/variation
Rgcc -/-
tissue
Lung

Sequenced DNA Library

library_strategy
Bisulfite-Seq
library_source
GENOMIC
library_selection
RANDOM
library_construction_protocol
Tissues were removed, flash frozen in LN2, and DNA was isolated using Tri-reagent. Illumina TruSeq RNA Sample Prep Kit (Illumina RS-122-2001/2) was used to construct sequencing libraries from double stranded amplicons of Bisulfite treated DNA. Illumina protocol

Sequencing Platform

instrument_model
Illumina MiSeq

mm10

Number of total reads
30202
Reads aligned (%)
100.0
Coverage rate (×)
0.0
Number of hyper MRs
1 (qval < 1E-05)

mm9

Number of total reads
30202
Reads aligned (%)
100.0
Coverage rate (×)
0.0
Number of hyper MRs
1 (qval < 1E-05)

Base call quality data from DBCLS SRA