Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
46C ES cells
cell type
46C ES cells
knock in
Atad2 TAP-tag
chip antibody
Anti-HA and anti-Flag

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The DNA was precipitated with ethanol in the presence of glycogen, and solubilized in water. The purified DNA was size-selected at 150 base pairs and processed for sequencing onto an Illumina analyzer Approximately 10ng of IP DNA from each samples were used for library preparation using the TruSeq ChIP Sample Prep protocol (Illumina) as described in the TruSeq ChIP Sample Preparation Data Sheet (Illumina).

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
17894808
Reads aligned (%)
97.7
Duplicates removed (%)
9.4
Number of peaks
216 (qval < 1E-05)

mm9

Number of total reads
17894808
Reads aligned (%)
97.5
Duplicates removed (%)
9.5
Number of peaks
264 (qval < 1E-05)

Base call quality data from DBCLS SRA