Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Blood

Cell type

Th1 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Sample

source_name

primary CD4+ T cells from spleen and lymph nodes

strain

B6.129S6-Tbx21<tm1Glm>/J (TbetKO)

genetic background

B6.129S6

cell type

in vitro polarized T helper1 cells for 7 days

passages

FACS sorted naive CD4+T cells cultured in vitro for 7 days under Th1 condition and restimulated with plate-bound CD3/28 +IL12 for 2 hrs

chip antibody

H3K36me3

chip antibody manufacturer

Abcam

Sequenced DNA Library

library_name

GSM836123: H3K36me3 TbetKO Th1

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

28520739

Reads aligned (%)

95.5

Duplicates removed (%)

25.7

Number of peaks

34455 (qval < 1E-05)

mm9

Number of total reads

28520739

Reads aligned (%)

95.3

Duplicates removed (%)

25.7

Number of peaks

34490 (qval < 1E-05)