Antigen

Antigen Class

TFs and others

Antigen

AR

Cell type

Cell type Class

Breast

Cell type

MDA-MB-453

Primary Tissue

Breast

Site of Extraction

Effusion, Pericardial

Tissue Diagnosis

Carcinoma

Sample

source_name

MDA-MB453_AR_siNON_R1881

origin

Metastatic carcinoma of the breast

cell line

MDA-MB453 breast cancer cell line

transfected with

control siRNA (siNON, Non-targeting pool)

treated with

10 nM R1881 for 2hrs

chip antibody

rabbit antiserum against AR

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were derived from sonicated nuclei and AR- and PIAS1-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocol, as utilized at the Functional Genomics Unit, Helsinki, Finland.

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

40290828

Reads aligned (%)

92.2

Duplicates removed (%)

26.3

Number of peaks

31006 (qval < 1E-05)

hg19

Number of total reads

40290828

Reads aligned (%)

91.7

Duplicates removed (%)

27.4

Number of peaks

31079 (qval < 1E-05)