Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Bone

Cell type

Chondrosarcoma

MeSH Description

A slowly growing malignant neoplasm derived from cartilage cells, occurring most frequently in pelvic bones or near the ends of long bones, in middle-aged and old people. Most chondrosarcomas arise de novo, but some may develop in a preexisting benign cartilaginous lesion or in patients with ENCHONDROMATOSIS. (Stedman, 25th ed)

Sample

source_name

RCS cells

cell type

Rat chondrosarcoma cell

chip-seq antibody

H3K4me3 (ab8580, Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RCS cell lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies. ChIP-seq DNA libraries wereprepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

rn6

Number of total reads

13956260

Reads aligned (%)

97.1

Duplicates removed (%)

18.9

Number of peaks

24856 (qval < 1E-05)