Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP63

Cell type

Cell type Class
Breast
Cell type
SUM 159PT
Primary Tissue
Breast
Tissue Diagnosis
Anaplastic Carcinoma

Attributes by original data submitter

Sample

source_name
SUM159 Cells ITGB4-Low
parental cell line
SUM159
subline
SUM159 Cells ITGB4-Low
cell type
mammary carcinoma cells
chip antibody
p63 (Cell Signalling 13109S)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with indicated antibody. Libraries were prepared according to Swift Biosciences instructions accompanying the ACCEL-NGS® 2S DNA LIBRARY KIT (Part# 20024) using primers and amplification of libraries from KAPA Biosystems HiFi library amp Kit (Part# KK2611).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
44078262
Reads aligned (%)
87.4
Duplicates removed (%)
49.9
Number of peaks
4043 (qval < 1E-05)

hg19

Number of total reads
44078262
Reads aligned (%)
87.2
Duplicates removed (%)
50.3
Number of peaks
4054 (qval < 1E-05)

Base call quality data from DBCLS SRA