GSM5242763: GIST-T1 DOT1L VTP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
DOT1L
Cell type
Cell type Class
Digestive tract
Cell type
GIST-T1
NA
NA
Attributes by original data submitter
Sample
source_name
KIT-dependent GIST cell line GIST-T1
cell line
GIST-T1
cell type
Gastrointestinal stromal tumor (GIST)
antibody
DOT1L, Cell Signaling Technology Cat# 77087
technique
ChIP-seq
target
Endogenous DOT1L
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries for single-end 75 bp sequencing on an Illumina NextSeq 500 or paired-end 37 bp sequencing on an Illumina NextSeq 550 were prepared with a ThruPLEX DNA-seq Kit (Takara).