GSM5237159: ChIP-MCF7 BRD4; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
BRD4
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Cell line MCF-7
cell line
MCF-7
chip antibody
BRD4
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq assay,the chromatin DNA precipitated by antibodies against BRD4, LSD1, MTA3, or H3K4me1. Then the DNA was purified with the QIAquick PCR Purification Kit. For RNA-seq assay, total RNAs were extracted from MCF-7 and MDA-MB-231 cells that were untreated or treated with JQ1 for 4 weeks using Trizol reagent. RNA and DNA libraries were prepared for sequencing using standard Illumina protocols