Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

hESCs

cell type

human embryonic stem cells (hESCs)

passages

p55

reprogramming method

None

clone

None

chip antibody

H3K4me3 (Active Motif, 39159)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA-seq:mRNA was extracted using the miRNEASY kit from Qiagen ChIP-seq:Cells were crosslinked with formalin RNA-seq: Ovation® RNA-Seq System V2 kit (NuGEN) and NEBNext DNA Library Prep Master Mix Set for Illumina ChIP-seq: NEBNext DNA Library Prep Master Mix Set for Illumina or NEBNext® Ultra™ DNA Library Prep

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

13192475

Reads aligned (%)

99.2

Duplicates removed (%)

5.9

Number of peaks

25937 (qval < 1E-05)

hg19

Number of total reads

13192475

Reads aligned (%)

99.0

Duplicates removed (%)

5.9

Number of peaks

25819 (qval < 1E-05)