Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXO1

Cell type

Cell type Class
Uterus
Cell type
Endometrial stromal cells
NA
NA

Attributes by original data submitter

Sample

source_name
endometrial stromal cells
cell type
primary cells
passages
2-4
health status
healthy, proliferative phase
antibody
FOXO1
antibody catalog
H-128/sc-11350

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and DNA complexes were isolated with antibody. ChIP and Input DNAs were prepared for amplification by converting overhangs into phosphorylated blunt ends and adding an adenine to the 3 -ends. Illumina genomic adapters were ligated and the sample was size-fractionated (200-300 bp) on a 2% agarose gel. After a final PCR amplification step (18 cycles), the resulting DNA libraries were quantified and sequenced on HiSeq 2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
40583067
Reads aligned (%)
45.6
Duplicates removed (%)
47.1
Number of peaks
34639 (qval < 1E-05)

hg38

Number of total reads
40583067
Reads aligned (%)
46.5
Duplicates removed (%)
46.3
Number of peaks
34747 (qval < 1E-05)

Base call quality data from DBCLS SRA