Antigen

Antigen Class

TFs and others

Antigen

RUNX1

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Leukemia cell line (THP1)

cell type

Paediatric monocytic cell line derived from AML M5 with t(9;11)(q21;q23) translocation

chip antibody

Abcam ab23980

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (EZH2). For reference normalisation 10% S2 cells were spiked in prior to sonication. Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

18044812

Reads aligned (%)

91.0

Duplicates removed (%)

2.0

Number of peaks

10706 (qval < 1E-05)

hg19

Number of total reads

18044812

Reads aligned (%)

90.1

Duplicates removed (%)

2.1

Number of peaks

10715 (qval < 1E-05)