Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Adipocyte

Cell type

Adipose-Derived Mesenchymal Stem Cells

NA

NA

Sample

source_name

adipocyte-derived stem cells (hADSCs)

chip_antibody

K9me3

o2 concentration

21%

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The cells were lysed by radioimmunoprecipitation assay (RIPA) buffer and the cell lysates were collected by centrifugation. Immunoprecipitated DNA was isolated with selected antibodies. Libraries were prepared according to Illumina's instructions. Briefly, 10 ng of DNA was end-repaired using a combination of Klenow polymerase and T4 polynucleotide kinase to yield a protruding 3-d'A' overhang. After adapter ligation, DNA was PCR amplified with Illumina primers for 18 cycles and library fragments of 100~500 bp were band isolated from an agarose gel. The purified DNA was captured on agilent bioanalyzer. Libraries were sequencedHiSeq 2000 platform following the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

22255250

Reads aligned (%)

97.5

Duplicates removed (%)

2.2

Number of peaks

1739 (qval < 1E-05)

hg19

Number of total reads

22255250

Reads aligned (%)

95.0

Duplicates removed (%)

5.0

Number of peaks

461 (qval < 1E-05)