Antigen

Antigen Class

TFs and others

Antigen

BEAF-32

Cell type

Cell type Class

Cell line

Cell type

S2

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

S2 cells

cell line

S2 cells

chip antibody

BEAF-33

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody Chromatin immobilization and barcoding performed as previously described (Gutin, J. et al. Fine-Resolution Mapping of TF Binding and Chromatin Interactions. Cell Rep. 22, 2797–2807 (2018).)

Sequencing Platform

instrument_model

NextSeq 500

dm6

Number of total reads

5796722

Reads aligned (%)

93.8

Duplicates removed (%)

30.9

Number of peaks

4128 (qval < 1E-05)

dm3

Number of total reads

5796722

Reads aligned (%)

94.8

Duplicates removed (%)

28.4

Number of peaks

4575 (qval < 1E-05)