Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Embryo
Cell type
XEN cells
NA
NA

Attributes by original data submitter

Sample

source_name
Extraembryonic endoderm stem cell line1
cell type
extraembryonic endoderm stem cell
treatment
Input control XEN cell line1 in standard maintenance media

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitation was performed on 1-2 x107 XEN cells as described (Vokes et al., 2007) no primary antibody control. Sonication was performed using a Covaris sonicator. Indexed libraries were prepared using the Illumina TruSeq ChIP Sample Preparation Kit according to the standard protocol. The libraries were sequenced on an Illumina HiSeq2000 with 50bp paired-end reads.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
69473051
Reads aligned (%)
98.0
Duplicates removed (%)
9.7
Number of peaks
363 (qval < 1E-05)

mm9

Number of total reads
69473051
Reads aligned (%)
97.9
Duplicates removed (%)
10.2
Number of peaks
400 (qval < 1E-05)

Base call quality data from DBCLS SRA