GSM1697712: G6L1 IP control 36h+d Line1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Mouse embryonic stem cell line1
cell type
embryonic stem cell
treatment
Input control in embryonic stem cell line1 36h following Gata6 induction
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitation was performed on 1-2 x107 cells as described (Vokes et al., 2007) no primary antibody control. Sonication was performed using a Misonix 4000 (28 cycles: 15 seconds on and 45 seconds off at an intensity of 70%) with a Microtip Probe (Misonix, Farmingdale, NY). Indexed libraries were prepared using the Illumina TruSeq ChIP Sample Preparation Kit according to the standard protocol. The libraries were sequenced on an Illumina HiSeq2000 with 50bp paired-end reads.