GSM5196484: TFAP2A ChIP-seq replicate2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TFAP2A
Cell type
Cell type Class
Embryo
Cell type
HEPM
Primary Tissue
Embryo
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Human embryonal palatal mesenchyme cells (HEPM cells)
cell type
Human embryonal palatal mesenchyme cells (HEPM cells)
chip antibody
TFAP2A polyclonal antibody ((ab52222, abcam®, Great Britain)
treatment
treated with antibody overnight
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
0.005 µg of polyclonal ChIP Grade TFAP2A antibody (ab52222, abcam®, Great Britain) were added to 25 µg of DNA. The treated samples incubated with rotation at 4°C overnight. Protein G magnetic beads were used to pull down the fragments and removed by incubation with ChIP elution buffer on a shaking thermomixer at 65°C for 30 min. To reverse DNA-crosslinking, the supernatant was treated with NaCl and Proteinase K overnight and purified using spin column tubes (provided in the kit). The NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs® GmbH, USA) was used according to the manufacturer's protocol. AmPure XP beads (Beckman Coulter™, USA) were used for clean up steps. No size selection was performed. Samples were equimolarly pooled. The library quality was controlled on an Agilent High Sensitivity D1000 system and samples were diluted to a final concentration of 2 nM. The replicates were sequenced twice on an Illumina MiSeq v2, yielding ~ 20 million 2x250bp paired end reads for each sample (ChIP and input control). Transcription factor ChIP-Seq