The hollow, muscular organ that maintains the circulation of the blood.
Attributes by original data submitter
Sample
source_name
heart tissue
age
13w
strain
Dahl salt sensitive
sample group
treatment [high-salt diet with 0.25 mg/kg of chaetocin treatment, HS (+)]
tissue
heart
chip-antibody
H3K4me3
chip-antibody vendor
abcam
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were extracted from one gram of crushed left ventricular tissue per group, cross-linked with 0.5% formaldehyde, sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions (TruSeq ChIP Sample Preparation Guide Rev. A) accompanying TruSeq ChIP Sample Prep Kit. Briefly, DNA were blunted, phosphorylated, protruded with 3- 'A' base, and ligated with adapters. After adapter ligation DNA was isolated from an agarose gel and amplified with PCR. The DNA was captured for cluster generation. Libraries were sequenced on the HiSeq 2000 following the manufacturer's protocols.