GSM5176711: LNCaP OGT Si2 INPUT; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TP53
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
LNCaPs
agent
OGT Si2
chip antibody
p53 (sc-126)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After 24h nutlin-3a treatment or 3 days post transfection, cells were fixed with 1% formaldehyde and chromatin prepared by sonication in Bioruptor plus. Fragmentation efficiency was analysed using fragment analyser and ChIP carried out using diagenode iDeal ChIP-seq kit for transcription factors (Cat# C01010170). Libraries were prepared using Diagenode MicroPlex Library Preparation Kit v2 (Cat# C05010014) and purified and purified/size selected using Agencourt® AMPure® XP beads (Beckman Coulter, Cat. No. A63881)