Antigen

Antigen Class

TFs and others

Antigen

twi

Cell type

Cell type Class

Embryo

Cell type

2-4h embryos

NA

NA

Sample

source_name

tl10b embryos

developmental stage

2-4 hr after egg deposition

genotype/variation

tl10b

chip antibody

Custom Covance Twi (aa 340-490)

barcode kit

NEB-next-ChIP

library kit

NEB-next-ChIP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed according to He et al. (2011) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

23833167

Reads aligned (%)

97.1

Duplicates removed (%)

18.5

Number of peaks

7769 (qval < 1E-05)

dm3

Number of total reads

23833167

Reads aligned (%)

97.4

Duplicates removed (%)

15.4

Number of peaks

7144 (qval < 1E-05)