Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
twi

Cell type

Cell type Class
Embryo
Cell type
2-4h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
tl10b embryos
developmental stage
2-4 hr after egg deposition
genotype/variation
tl10b
chip antibody
Custom Covance Twi (aa 340-490)
barcode kit
NEB-next-ChIP
library kit
NEB-next-ChIP

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed according to He et al. (2011) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
23833167
Reads aligned (%)
82.4
Duplicates removed (%)
15.4
Number of peaks
7144 (qval < 1E-05)

dm6

Number of total reads
23833167
Reads aligned (%)
79.1
Duplicates removed (%)
18.5
Number of peaks
7769 (qval < 1E-05)

Base call quality data from DBCLS SRA