Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryo

Cell type

2-4h embryos

NA

NA

Sample

source_name

gd7 embryos

developmental stage

2-4 hr after egg deposition

genotype/variation

gd7

chip antibody

H3K27me3 (Active Motif Carlsbad, CA, catalog #39155)

barcode kit

NEB-next-ChIP

library kit

NEB-next-ChIP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed according to He et al. (2011) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

24535827

Reads aligned (%)

96.6

Duplicates removed (%)

22.9

Number of peaks

5053 (qval < 1E-05)

dm3

Number of total reads

24535827

Reads aligned (%)

96.9

Duplicates removed (%)

20.8

Number of peaks

5080 (qval < 1E-05)