GSM5155187: shDouble R1881; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
LNCaP prostate cancer cell line
cell line
LNCaP
genotype
shMAP3K7_puro, shCHD1_neo
treatment
1nM R1881, 4 hours
passage
52
chip antibody
AR N-20 (Santa Cruz #sc-816)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, lysates were sonicated, precleared, and incubated with 10ug antibody (AR N-20) and agarose beads. Aliquots of precleared lysates were taken as input controls before antibody incubation. Beads were extensivley washed and protein-antibody complexes were eluted and reverse cross-linked. Supernatants were treated with Proteinase K, RNAase A. DNA was purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and meausured with the Qubit dsDNA HS kit (Invitrogen). 1 ng of ChIP or input DNA per sample was used for library construction following NuGEN DNA-Seq library preperpation instructions.