Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Neural

Cell type

Glioblastoma

MeSH Description

A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.

Sample

source_name

Gliobla

biomaterial_provider

Dr. Darrell Bigner at Duke University Medical Center

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

treatment

None

treatment description

No special treatment or protocol applies

antibody antibodydescription

mouse monoclonal, clone 8WG16. Antibody Target: POL2

antibody targetdescription

RNA Polymerase II

antibody vendorname

Covance

antibody vendorid

MMS-126R

controlid

wgEncodeEH001129

labversion

baseAlignCounts.pl v 1,fseq v 1.84, iff_H54,p-value cutoff: 0.05

replicate

1,2

antibody antibodydescription

mouse monoclonal, clone 8WG16

antibody targetdescription

RNA Polymerase II

antibody vendorname

Covance

antibody vendorid

MMS-126R

controlid

Gliobla/Input

labversion

align_on_cluster_bwa.pl v 1

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM822302: UT-A_ChipSeq_Gliobla_Pol2

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

57833903

Reads aligned (%)

63.5

Duplicates removed (%)

13.5

Number of peaks

20857 (qval < 1E-05)

hg19

Number of total reads

57833903

Reads aligned (%)

63.1

Duplicates removed (%)

14.1

Number of peaks

20971 (qval < 1E-05)