Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Digestive tract

Cell type

Jejunum

MeSH Description

The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum.

Sample

source_name

Jejunal epithelial cells

cell type

Jejunal epithelial cells

strain

Gata4FlagBio, C57BL/6

developmental stage

Adult

protocol used

BioChIPseq protocol was used according to He et al. (Curr Protoc Mol Biol. 2010 Oct;Chapter 21:Unit 21.20).

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

6 week old adult mice were dissected. Jejunal and ileal epithelial cells were subsequently extracted by using the adjusted Weisser method (Verzi et al. Dev Cell 2010 Nov 16;19(5):713-26.) NEB Next kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

50209525

Reads aligned (%)

93.2

Duplicates removed (%)

23.6

Number of peaks

22625 (qval < 1E-05)

mm9

Number of total reads

50209525

Reads aligned (%)

93.0

Duplicates removed (%)

23.6

Number of peaks

22659 (qval < 1E-05)