Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Bone Marrow Cells

MeSH Description

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Sample

source_name

Bone marrow

cell type

MLL1 flox/flox, ER-cre+/-, MAF9 transduced mouse bone marrow cells

agent

EtOH

antibody

H3K4me2 (Millipore, cat#07-030, Lot # 2142243)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP experiments were performed using 1E8 cells and 15µl of antibodies per ChIP according to standard protocols. Libraries were prepared with the Illumina TruSeq protocol according to the manufacturer’s protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

92286474

Reads aligned (%)

82.2

Duplicates removed (%)

50.1

Number of peaks

45092 (qval < 1E-05)

mm9

Number of total reads

92286474

Reads aligned (%)

82.2

Duplicates removed (%)

50.1

Number of peaks

45075 (qval < 1E-05)