Antigen

Antigen Class

TFs and others

Antigen

ATF4

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Leukemia cell line (THP1)

tissue

Leukemia cell line (THP1)

treatment

Cells cultured in RPMI for 72h

cell type

Paediatric monocytic cell line derived from AML M5 with t(9;11)(q21;q23) translocation

chip antibody

Cell Signaling Technology 11815

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS and fixed in 2mM DSG for 30 minutes and 1% FA for 30 minutes. Fixed Drosophila S2 cells were added at a 1:4 ratio (Dm:Hs) to allow reference normalization. Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

25299781

Reads aligned (%)

97.0

Duplicates removed (%)

11.9

Number of peaks

23537 (qval < 1E-05)

hg19

Number of total reads

25299781

Reads aligned (%)

96.1

Duplicates removed (%)

12.2

Number of peaks

23346 (qval < 1E-05)