Antigen

Antigen Class

TFs and others

Antigen

HIF1A

Cell type

Cell type Class

Neural

Cell type

SK-N-BE(2)

Primary Tissue

Brain

Site of Extraction

Bone Marrow

Tissue Diagnosis

Neuroblastoma

Sample

source_name

SK-N-BE(2) cell line

cell line

SK-N-BE(2) cell line

cell type

Neuroblastoma

treatment

1% oxygen

treatment length

48 hours

antibody

HIF-1alpha

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fixed chromatin was sheared for HIF-1alpha ChIP. ChIPed DNA was reverse-crosslinked and purified for sequencing Library was prepared with standard Illumina protocol by the University of Chicago Sequencing Core

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

26268468

Reads aligned (%)

95.6

Duplicates removed (%)

28.0

Number of peaks

1407 (qval < 1E-05)

hg19

Number of total reads

26268468

Reads aligned (%)

94.7

Duplicates removed (%)

28.8

Number of peaks

1223 (qval < 1E-05)