GSM5100368: CTCF ChIP-seq in iNeuron; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPSC derived neural cells
NA
NA
Attributes by original data submitter
Sample
source_name
iNeuron
cell type
neuron differentiated from iPSC
treatment
NT
chip antibody
6 ul, Millipore, Cat. No. 07-729
harvested day
day7
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde. Cells were sonicated, lysates clarified, and protein-DNA complexes immunoprecipitated. Libraries were quantified using with the KAPA Library Quantification Kit (Kapa Biosciences) and sequenced in a NextSeq 550 system (Illumina, 75 bp single end reads). libraries were prepared and sequenced following standard Illumina protocols