Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
LMO2

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
t(8;21) RUNX1-ETO AML
cell line
KASUMI-1 cell line
genotype
KASUMI-1_BETi
chip antibody
LMO2 (R&D, AF2726)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
x-linking was performed with 1% Formaldehyde. Lysates were clarified from sonicated nuclei and chromatin proteins were isolated with antibodies. ChIP-Seq libraries were prepared for sequencing using a TruSeq ChIP Sample Prep Kit (Illumina) and standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
28819829
Reads aligned (%)
97.8
Duplicates removed (%)
16.5
Number of peaks
5907 (qval < 1E-05)

hg19

Number of total reads
28819829
Reads aligned (%)
96.9
Duplicates removed (%)
18.1
Number of peaks
5874 (qval < 1E-05)

Base call quality data from DBCLS SRA