Antigen

Antigen Class

TFs and others

Antigen

RUNX1

Cell type

Cell type Class

Blood

Cell type

Kasumi-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

t(8;21) RUNX1-ETO AML

cell line

KASUMI-1 cell line

genotype

KASUMI-1_BETi

chip antibody

RUNX1 (Abcam, ab23980)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

x-linking was performed with 1% Formaldehyde. Lysates were clarified from sonicated nuclei and chromatin proteins were isolated with antibodies. ChIP-Seq libraries were prepared for sequencing using a TruSeq ChIP Sample Prep Kit (Illumina) and standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

24601534

Reads aligned (%)

66.1

Duplicates removed (%)

30.7

Number of peaks

9707 (qval < 1E-05)

hg19

Number of total reads

24601534

Reads aligned (%)

65.5

Duplicates removed (%)

32.3

Number of peaks

9719 (qval < 1E-05)