Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

T-47D

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

T47D_ER_3hr_PG

cell line

T47D

cell type

ER+ breast cancer cell line

condition

PG

chip antibody

ERa Antibody (HC-20)

chip antibody vendor

Santa Cruz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 50bp sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendations

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg19

Number of total reads

52917782

Reads aligned (%)

90.7

Duplicates removed (%)

6.4

Number of peaks

1555 (qval < 1E-05)

hg38

Number of total reads

52917782

Reads aligned (%)

93.1

Duplicates removed (%)

4.7

Number of peaks

1794 (qval < 1E-05)