Antigen

Antigen Class

TFs and others

Antigen

SPI1

Cell type

Cell type Class

Blood

Cell type

MV-4-11

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

MV411 cell line

cell line

MV411 leukemia cell line

treatment

CRYBMIM peptide

time

1 hr

chip antibody

Cell Signaling #2266 Anti-PU.1, rabbit polyclonal

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer's recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

35670543

Reads aligned (%)

98.5

Duplicates removed (%)

6.7

Number of peaks

504 (qval < 1E-05)

hg19

Number of total reads

35670543

Reads aligned (%)

97.7

Duplicates removed (%)

6.9

Number of peaks

342 (qval < 1E-05)