Antigen

Antigen Class

TFs and others

Antigen

CEBPD

Cell type

Cell type Class

Others

Cell type

Fibroblasts

NA

NA

Sample

source_name

amnion fibroblasts

cell type

fibroblasts

treatment

untreated

chip antibody

CEBPdelta (GeneTex, GTX115047)

tissue

amnion

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protien-DNA complexes were isolated with CEBPδ antibody. 5 ng of purified ChIP DNA were used to generate the sequencing library using a NEB kit

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

22449056

Reads aligned (%)

83.7

Duplicates removed (%)

12.3

Number of peaks

37815 (qval < 1E-05)

hg19

Number of total reads

22449056

Reads aligned (%)

83.0

Duplicates removed (%)

12.4

Number of peaks

37240 (qval < 1E-05)