Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SP1

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
hepatocellular carcinoma
antibody antibodydescription
Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target: SP1
antibody targetdescription
SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-17824
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL1400,SL1401
labexpid
SL1056,SL1194
replicate
1,2
antibody antibodydescription
Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA.
antibody targetdescription
SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-17824
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL1400
labexpid
SL1056
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803507: HudsonAlpha_ChipSeq_HepG2_SP1_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
70960591
Reads aligned (%)
55.3
Duplicates removed (%)
10.5
Number of peaks
30153 (qval < 1E-05)

hg38

Number of total reads
70960591
Reads aligned (%)
56.5
Duplicates removed (%)
9.5
Number of peaks
30212 (qval < 1E-05)

Base call quality data from DBCLS SRA