Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TAF7

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal IgG, raised against recombinant TAF II p55 (TAF7) of human origin. Antibody Target: TAF7
antibody targetdescription
The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II (RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-101167
controlid
SL1398
labexpid
SL3062,SL2697
replicate
1,2
softwareversion
MACS
antibody
TAF7_(SC-101167)
antibody antibodydescription
Mouse monoclonal IgG, raised against recombinant TAF II p55 (TAF7) of human origin.
antibody targetdescription
The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II (RefSeq).
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-101167
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1398
labexpid
SL2697
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803501: HudsonAlpha_ChipSeq_H1-hESC_TAF7_(SC-101167)_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
122283692
Reads aligned (%)
58.9
Duplicates removed (%)
24.0
Number of peaks
17497 (qval < 1E-05)

hg38

Number of total reads
122283692
Reads aligned (%)
60.5
Duplicates removed (%)
22.6
Number of peaks
17872 (qval < 1E-05)

Base call quality data from DBCLS SRA