Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Neural
Cell type
SK-N-SH
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
SK-N-SH_RA
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
neuroblastoma cell line, treatment: differentiated with retinoic acid, (Biedler, et al. Morphology and Growth, Tumorigenicity, and Cytogenetics of Human Neuroblastoma Cells in Continuous Culture. Cancer Research 33, 2643-2652, November 1973.)
antibody antibodydescription
Rabbit polyclonal. Antibody Target: RAD21
antibody targetdescription
Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14
antibody vendorname
abcam
antibody vendorid
ab992
controlid
SL2905
labexpid
SL2915,SL2907
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Rabbit polyclonal
antibody targetdescription
Synthetic peptide (Human) conjugated to KLH - which represents a portion of human Rad21 encoded within exon 14
antibody vendorname
Abcam
antibody vendorid
ab992
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL2905
labexpid
SL2907
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803497: HudsonAlpha_ChipSeq_SK-N-SH_RA_Rad21_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
68805676
Reads aligned (%)
56.9
Duplicates removed (%)
34.5
Number of peaks
95343 (qval < 1E-05)

hg38

Number of total reads
68805676
Reads aligned (%)
57.5
Duplicates removed (%)
34.0
Number of peaks
95572 (qval < 1E-05)

Base call quality data from DBCLS SRA