Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

GM12892

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed

Sample

source_name

GM12892

biomaterial_provider

Coriell

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed

antibody antibodydescription

Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target: POL2

antibody targetdescription

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

antibody vendorname

abcam

antibody vendorid

ab5408

controlid

SL818

labexpid

SL946,SL911

replicate

1,2

antibody

Pol2-4H8

antibody antibodydescription

Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade

antibody targetdescription

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

antibody vendorname

Abcam

antibody vendorid

ab5408

treatment

None

treatment description

No special treatment or protocol applies

protocol

v041610.2

protocol description

2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)

controlid

SL818

labexpid

SL911

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803490: HudsonAlpha_ChipSeq_GM12892_Pol2-4H8_v041610.2

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

52723486

Reads aligned (%)

76.3

Duplicates removed (%)

8.1

Number of peaks

28962 (qval < 1E-05)

hg19

Number of total reads

52723486

Reads aligned (%)

74.3

Duplicates removed (%)

7.2

Number of peaks

29940 (qval < 1E-05)