Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

GM12878

Tissue

blood

Lineage

mesoderm

Description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Sample

source_name

GM12878

biomaterial_provider

GM12878

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell description

B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

antibody antibodydescription

Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target: POL2

antibody targetdescription

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

antibody vendorname

abcam

antibody vendorid

ab5408

protocol description

one 15-cycle round of PCR (Myers)

controlid

SL516,SL517

labexpid

SL748,SL847

labversion

SL748,SL847

replicate

1,2

softwareversion

MACS

antibody vendorname

Abcam

antibody vendorid

ab5408

treatment

None

treatment description

No special treatment or protocol applies

protocol

PCR1x

protocol description

1-cycle of PCR (Myers)

controlid

SL517

labexpid

SL748

replicate

1

replicate description

tier 1

Sequenced DNA Library

library_name

GSM803485: HudsonAlpha_ChipSeq_GM12878_Pol2-4H8_PCR1x

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer

hg38

Number of total reads

88933754

Reads aligned (%)

71.0

Duplicates removed (%)

15.7

Number of peaks

31159 (qval < 1E-05)

hg19

Number of total reads

88933754

Reads aligned (%)

70.1

Duplicates removed (%)

15.6

Number of peaks

32305 (qval < 1E-05)