Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SRF

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Rabbit polyclonal. Antibody Target: SRF
antibody targetdescription
Serum response transcription factor
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-335
controlid
SL1613
labexpid
SL3578,SL3827
replicate
1,2
softwareversion
MACS
antibody antibodydescription
Rabbit polyclonal
antibody targetdescription
Serum response transcription factor
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-335
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL1613
labexpid
SL3578
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803477: HudsonAlpha_ChipSeq_GM12878_SRF_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
63961294
Reads aligned (%)
48.0
Duplicates removed (%)
37.1
Number of peaks
15857 (qval < 1E-05)

hg38

Number of total reads
63961294
Reads aligned (%)
49.2
Duplicates removed (%)
36.5
Number of peaks
15102 (qval < 1E-05)

Base call quality data from DBCLS SRA