Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TAF1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Mouse monoclonal (p250). Antibody Target: TAF1
antibody targetdescription
TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-735
controlid
SL969
labexpid
SL964,SL853
replicate
1,2
cell sex
M
antibody
TAF1
antibody antibodydescription
Mouse monoclonal (p250)
antibody targetdescription
TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa. Also designated TAF250.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-735
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL969
labexpid
SL853
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803450: HudsonAlpha_ChipSeq_H1-hESC_TAF1_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
40640767
Reads aligned (%)
70.3
Duplicates removed (%)
12.3
Number of peaks
29494 (qval < 1E-05)

hg38

Number of total reads
40640767
Reads aligned (%)
71.7
Duplicates removed (%)
11.2
Number of peaks
29508 (qval < 1E-05)

Base call quality data from DBCLS SRA