Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TEAD1

Cell type

Cell type Class
Lung
Cell type
MSTO-211H
Primary Tissue
Pleura
Tissue Diagnosis
Mesothelioma

Attributes by original data submitter

Sample

source_name
MSTO human cell line
cell line
MSTO
antibody
TEAD1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked in 1% formaldehyde for 10 minutes at room temperature after which the reaction was stopped by addition of 0.125M glycine. Cells were lysed and harvested in ChIP buffer (100 mM Tris at pH 8.6, 0.3% SDS, 1.7% Triton X-100 and 5 mM EDTA) and the chromatin disrupted by sonication using a Diagenode Bioruptor sonicator UCD-200 to obtain fragments of 200-500 bp in size. Suitable amounts of chromatin were incubated with specific antibodies overnight. Antibodies used are: YAP (Cell Signaling – Cat. # 14074), TEAD1 (BD – Cat. # 610922), H3K27ac (Abcam – Cat. # ab4729) Libraries were generated using NEBNext Ultra DNA Library Prep Kit for Illumina and barcoded using NEBNext Multiplex Oligos for Illumina according to manufacturer recommendation.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
39562937
Reads aligned (%)
87.4
Duplicates removed (%)
32.9
Number of peaks
18755 (qval < 1E-05)

hg19

Number of total reads
39562937
Reads aligned (%)
87.0
Duplicates removed (%)
34.0
Number of peaks
18759 (qval < 1E-05)

Base call quality data from DBCLS SRA