Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SP2

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping near the N-terminus of SP2 of human origin. Antibody Target: SP2
antibody targetdescription
This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-643
controlid
SL1396
labexpid
SL2951,SL3361
replicate
1,2
softwareversion
MACS
antibody
SP2_(SC-643)
antibody antibodydescription
Rabbit polyclonal IgG, epitope mapping near the N-terminus of SP2 of human origin.
antibody targetdescription
This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-643
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.2
protocol description
2x10^7 cells, fragmentation by probe-in sonicator, one 15-cycle round of PCR (Myers)
controlid
SL1396
labexpid
SL2951
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803402: HudsonAlpha_ChipSeq_K562_SP2_(SC-643)_v041610.2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
47692081
Reads aligned (%)
58.1
Duplicates removed (%)
29.1
Number of peaks
3841 (qval < 1E-05)

hg38

Number of total reads
47692081
Reads aligned (%)
59.0
Duplicates removed (%)
28.4
Number of peaks
3741 (qval < 1E-05)

Base call quality data from DBCLS SRA