Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCFL

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
antibody antibodydescription
Rabbit polyclonal IgG, epitope corresponding to amino acids 91-210 mapping within an internal region of BORIS(CTCFL) of human origin. Antibody Target: CTCFL
antibody targetdescription
CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-98982
controlid
SL3980,SL2455
labexpid
SL3189,SL3972
replicate
1,2
softwareversion
MACS
antibody
CTCFL_(SC-98982)
antibody antibodydescription
Rabbit polyclonal IgG, epitope corresponding to amino acids 91-210 mapping within an internal region of BORIS(CTCFL) of human origin.
antibody targetdescription
CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. (provided by RefSeq)
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-98982
treatment
None
treatment description
No special treatment or protocol applies
protocol
v041610.1
protocol description
2x10^7 cells, fragmentation by bioruptor, one 15-cycle round of PCR (Myers)
controlid
SL2455
labexpid
SL3189
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803401: HudsonAlpha_ChipSeq_K562_CTCFL_(SC-98982)_v041610.1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
46434439
Reads aligned (%)
45.2
Duplicates removed (%)
46.1
Number of peaks
15889 (qval < 1E-05)

hg38

Number of total reads
46434439
Reads aligned (%)
46.3
Duplicates removed (%)
45.1
Number of peaks
15843 (qval < 1E-05)

Base call quality data from DBCLS SRA