Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pancreas
Cell type
PANC-1
Tissue
Pancreas/Duct
Disease
Epithelioid Carcinoma

Attributes by original data submitter

Sample

source_name
PANC-1
biomaterial_provider
ATCC
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
pancreatic carcinoma, (PMID: 1140870) PANC-1 was established from a pancreatic carcinoma, which was extracted via pancreatico-duodenectomy specimen from a 56-year-old Caucasian individual. Malignancy of this cell line was verified via in vitro and in vivo assays.
protocol description
a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)
controlid
SL523,SL117
labexpid
SL523,SL117
labversion
SL523,SL117
replicate
1,2
cell lineage
endoderm
cell sex
M
antibody
RevXlinkChromatin
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR2x
protocol description
2-cycles of PCR (Myers)
controlid
SL117
labexpid
SL117
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803394: HudsonAlpha_ChipSeq_PANC-1_RevXlinkChromatin_PCR2x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
126146676
Reads aligned (%)
42.9
Duplicates removed (%)
40.0
Number of peaks
3792 (qval < 1E-05)

hg19

Number of total reads
126146676
Reads aligned (%)
42.4
Duplicates removed (%)
41.1
Number of peaks
2748 (qval < 1E-05)

Base call quality data from DBCLS SRA