Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
IRF4

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Goat polyclonal IgG, epitope mapping at the C-terminus of IRF-4 of mouse origin. Antibody Target: IRF4
antibody targetdescription
The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family. It functions as a lymphocyte specific transcription factor and negatively regulates TLR signaling by selectively competing with IRF5. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma (RefSeq).
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-6059
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL516,SL517
labexpid
SL951,SL838
labversion
8,7
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-6059
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL516
labexpid
SL838
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803390: HudsonAlpha_ChipSeq_GM12878_IRF4_(SC-6059)_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
53089739
Reads aligned (%)
71.7
Duplicates removed (%)
4.3
Number of peaks
29170 (qval < 1E-05)

hg38

Number of total reads
53089739
Reads aligned (%)
73.6
Duplicates removed (%)
3.8
Number of peaks
28482 (qval < 1E-05)

Base call quality data from DBCLS SRA