Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SP1

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878
biomaterial_provider
GM12878
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus
antibody antibodydescription
Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target: SP1
antibody targetdescription
SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter.
antibody vendorname
Santa Cruz Biotechnology
antibody vendorid
sc-17824
protocol description
one 15-cycle round of PCR (Myers)
controlid
SL678,SL517
labexpid
SL846,SL746
labversion
SL846
replicate
1,2
softwareversion
MACS
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-17824
treatment
None
treatment description
No special treatment or protocol applies
protocol
PCR1x
protocol description
1-cycle of PCR (Myers)
controlid
SL517
labexpid
SL746
replicate
1
replicate description
tier 1

Sequenced DNA Library

library_name
GSM803363: HudsonAlpha_ChipSeq_GM12878_SP1_PCR1x
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
132434296
Reads aligned (%)
45.2
Duplicates removed (%)
14.8
Number of peaks
41839 (qval < 1E-05)

hg38

Number of total reads
132434296
Reads aligned (%)
46.3
Duplicates removed (%)
14.0
Number of peaks
40074 (qval < 1E-05)

Base call quality data from DBCLS SRA